Trehalose Inhibits Inflammatory Cytokine Production

Comments by J. C. Spencer

A research paper on the sugar trehalose is to be published in the August 2009 issue of the Oral Biology, a multidisciplinary journal of oral and cranofacial sciences that shows trehalose inhibits inflammatory conditions in mice. This University science paper verifies that additional research is needed to determine if trehalose inhibits inflammation in humans. This paper adds to the many published papers on the structure function of trehalose.

The Endowment for Medical Research has conducted Nutritional Pilot Surveys using trehalose. Also, significant educational and research information concerning trehalose can be found at www.endowmentmed.org and at www.DiabeticHope.com . You may request Evaluation Forms or they may be downloaded from the Home Page.

Please submit information if you have experienced health benefits from using the sugar trehalose. Your participation may help many others in the future.

Now for the Abstract
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Trehalose inhibits inflammatory cytokine production by protecting I�B-� reduction in mouse peritoneal macrophages

Abstract

Objective
The aim of this study was to examine whether trehalose, a disaccharide, could inhibit Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS)-enhanced production of inflammatory cytokines in mouse peritoneal macrophages.

Design
Mouse peritoneal macrophages were treated with trehalose and stimulated with P. gingivalis LPS. Interleukin-1� (IL-1�) and tumour necrosis factor-� (TNF-�) levels in the culture supernatant were measured by ELISA. The mRNA levels of the cytokines in macrophages were analysed by semi-quantitative RT-PCR. DNA and protein synthesis were measured by incorporation of [3H] thymidine or [14C] praline into mouse peritoneal macrophages. I�B-� reductions were assessed by western blot.

Results
Treatment with trehalose suppressed LPS-induced IL-1� and TNF-� production and downregulated transcription of these cytokines. Furthermore, trehalose inhibited LPSinduced reduction of I�B-�. In addition, we also observed expression of the trehalose receptor (T1R3) in mouse peritoneal macrophages.

Conclusion
These results may suggest that trehalose inhibits LPS-induced production of IL-1� and TNF-� in mouse peritoneal macrophages by inhibiting degradation of I�B-� via the trehalose receptor T1R3.

Kahoru Tayaa(a), Kimiharu Hiroseb(b), Setsuo Hamadaa(a)

a Division of Dental Pharmacology, Department of Oral Medical Science, Ohu University School of Dentistry, Koriyama, Fukushima 963-8611, Japan
b Division of Preventive Dentistry, Department of Oral Health, Ohu University School of Dentistry, Koriyama, Japan

Oral Biology, a multidisciplinary journal of oral and cranofacial sciences
Volume 54, Issue 8, Pages 749-756 (August 2009)

Corresponding author. Tel.: +81 24 932 8982; fax: +81 24 932 8983.
PII: S0003-9969(09)00119-8
doi:10.1016/j.archoralbio.2009.05.003
� 2009 Elsevier Ltd. All rights reserved.

Keywords: Trehalose, Interleukin-1?, Tumour necrosis factor-?, I?B-?

The full text may be obtained from Oral Biology http://www.aobjournal.com/article/PIIS0003996909001198/abstract?rss=yes

www.endowmentmed.org